Klondike Hotel and Casino (also known as Klondike Inn) was a hotel and casino located on the Las Vegas Strip in Paradise, Nevada, in the United States. The property began as the Kona Kai Motel in 1962, and was purchased by Ralph Engelstad in 1969. The motel was sold to John Woodrum, who renamed it as the Klondike Inn in 1976. A casino was eventually added, and the Klondike became popular among local residents. In 2005, the Klondike was sold to Royal Palm Las Vegas, which planned to replace it with a casino and condo hotel resort known as Paramount Las Vegas. The Klondike closed in June 2006, and was demolished in March 2008. Royal Palm Las Vegas had difficulty obtaining financing for the Paramount project, and the land was put up for sale later in 2008. A Harley-Davidson dealership opened on the former Klondike property in 2014.

The hotel sat on 5.29 acres (2.14 ha) of land on Las Vegas Boulevard, between the Welcome to Fabulous Las Vegas sign and McCarran International Airport. The Klondike was the southernmost hotel on the Las Vegas Strip until its closure. Mandalay Bay is now the southernmost hotel. Woodrum also operated a sister property, the Klondike Sunset Casino, in nearby Henderson, from 1999 until his death in 2014.